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KMID : 0613820070170111601
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Journal of Life Science
2007 Volume.17 No. 11 p.1601 ~ p.1604
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Secretory Overexpression of β-Agarase in Bacillus subtilis and Antibacterial Activity of Enzymatic Products
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Jang Min-Kyung
Lee Ok-Hee
Lee Dong-Geun
Lee Sang-Hyeon
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Abstract
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The gene for β-agarase of an Agarivorans sp. JA-1 was expressed in Bacillus subtilis DB104, 168 and ISW1214 strains for mass-production. Among 3 host strains, B. subtilis ISW1214 secreted the highest amount of recombinant β-agarase with a specific activity of 201 U/mg and 360 mg of protein into culture broth. This was approximately 130-fold higher than the production in E. coli as an expression host. Recombinant enzyme produced neoagarooligosaccharides such as neoagarohexaose, neoagarotetraose, and neoagarobiose from agar. Produced neoagarooligosaccharides showed antibacterial activities against gram-negative E. coli and gram-positive B. subtilis at a concentration of 1.5%. These data suggest that neoagarooligosaccharides could be an useful preservative for food industry.
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KEYWORD
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β-agarase, antibacterial activity, neoagarooligosaccharides, secretory expression
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